Abstract
Abstract A microchip thermocycler, fabricated from silicon and Pyrex #7740 glass, is described. Usual resistive heating has been replaced by induction heating, leading to much simpler fabrication steps. Heating and cooling rates of 6.5 and 4.2 °C/s, respectively have been achieved, by optimising the heater dimensions and heating frequency (∼200 kHz). Four devices are mounted on a heater, resulting in low power consumption (∼1.4 W per device on the average). Using simple on–off electronic temperature control, a temperature stability within ∼0.2 °C is achieved. Features such as induction heating, good temperature control, battery operation, and low power consumption make the device suitable for portable applications, particularly in polymerase chain reaction (PCR) systems.
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