Abstract
In the current study, we report here the design, fabrication and functional verification of a novel, portable and automatic dual-readout device for carbamate pesticides detection applications with low detection limits. This device integrated a 3D printed, low cost and disposable microfluidic chip to deliver nanomaterials and pesticides samples. Based on the cross-responsive mechanism and the agglomeration effect of AuNPs, two model signals (fluorescence and color change, dual-readout) were achieved from the response between pesticides and sensing materials. Simultaneously, a novel and unique fluorescence difference patterns (spectra-fingerprint) were produced based on cross-responsive mechanism for specificity recognition. The difference maps including color difference image and fluorescence difference spectra presented good selectivity among carbamate pesticides (six kinds of carbamate pesticides were successfully distinguished) or other categories in low concentration. By use of this dual-signal model, the device could achieve the lowest detectable concentrations for carbamate pesticides at ppb level with the practicability, reproducibility and stability estimated. Importantly, with the features of integration and instrumentation, the detector could be further employed for the home-based testing and environmental monitoring.
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