Abstract
Abstract Preparation of trans-(2R,3S) methyl (4-methoxyphenyl)glycidate with enantiomeric excess (e.e.) of >99% has been carried out by enantioselective hydrolysis of the racemic glycidate ester by Lecitase® Ultra immobilized in macroporous gelatin organo-gel (gelozyme) in 47% yield. Effects of water content and particle size of the gel, different solvents, and flow rate of reaction medium on observed reaction velocity lead to optimum conditions. The reaction performed in a porous vessel bioreactor using toluene as preferred reaction medium, and gelatin-immobilized enzyme with average particle size of 1–1.2 mm, water content of 16.5% (w/w), and flow rate of 20 mL min−1, followed a typical Michaelis–Menten kinetics with apparent Vmax,app of 38 mM min−1 g−1 and apparent Km of 0.53 M. Theoretical considerations suggest that the bioreactor functions as a plug-flow reactor with complete recycle without serious pore diffusion and external mass transfer diffusion limitations. The immobilized enzyme can be used continuously for several recycles without significant loss of activity for at least two weeks.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
