Abstract
According to the WHO 2009 classification, dengue with warning signs is at the risk of developing severe form of dengue disease. One of the most important warning signs is plasma leakage, which can be a serious complication associated with higher morbidity and mortality. We report that the frequency of CD4+CD8+ double-positive (DP) T cells is significantly increased in patients at risk of developing plasma leakage. Transcriptomic analysis demonstrated that CD4+CD8+ DP cells were distinct from CD4+ Single Positive (SP) T cells but co-clustered with CD8+ SP cells, indicating a largely similar transcriptional profile. Twenty significant differentially expressed (DE) genes were identified between CD4+CD8+ DP and CD8+ SP cells. These genes encode OX40 and CCR4 proteins as well as other molecules associated with cell signaling on the cell surface (NT5E, MXRA8, and PTPRK). While comparing the profile of gene expression in CD4+CD8+ DP cells from patients with and without warning signs of plasma leakage, similar expression profile was observed, implying a role of CD4+CD8+ DP cells in plasma leakage through a quantitative increase rather than functional alteration. This study provided novel insight into the host immune response during the acute febrile phase of DENV infection and the role of CD4+CD8+ DP T cells in the pathogenesis of plasma leakage.
Highlights
Dengue fever (DF) is one of the major re-emerging infectious diseases and the most prevalent arthropod-borne viral disease in humans [1]
We report that a population of CD4+CD8+ double-positive (DP) T cells detectable in the peripheral blood is associated with risk of plasma leakage in dengue disease
Expansion of CD4+CD8+ DP T Cells Is Associated with Risk of Plasma Leakage in Dengue
Summary
Dengue fever (DF) is one of the major re-emerging infectious diseases and the most prevalent arthropod-borne viral disease in humans [1]. The 1997 WHO classification [3] distinguished four main categories of dengue disease: nonclassical DF, classical DF, dengue hemorrhagic fever (DHF), and dengue shock syndrome (DSS). The diagnosis of DHF required the presence of four criteria: fever, thrombocytopenia (
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
