Abstract
In this study, the purification and characterization of a polysaccharide-based bioflocculant BP50-2 of banana peel waste were investigated. BP50-2 was purified and identified through HPLC, XPS, TG, SEM, AFM, etc. The results showed that BP50-2 was a heteropolysaccharide composed of Mannose, Rhamnose, Glucuronic acid, Galacturonic acid, Glucose, Galactose, and Fucose at a molar ratio of 8.97:5.36:1.92:1.00:32.52:8.30:2.64, respectively. The MW of BP50-2 was 1.47×10 3 KDa. BP50-2 had high pH stability that maintained flocculation activity on kaolin suspension for a pH range from 3 to 11, and high-temperature stability ranged from room temperature to 90°C. BP50-2 was non-sensitive to cation and affected by HCl, EDTA, little affected by urea, which showed that the BP50-2 was non-cation dependent and its main binding mode with kaolin was ionic bond and contained a small amount of hydrogen bond. And flocculation mechanisms were discussed, which indicated that adsorption bridging was the main mechanism of the flocculation process of BP50-2-Kaolin or BP50-2-Mg2+-Kaolin. Moreover, BP50-2 had decolorization activity on RhB and removal activity of heavy metal ion, of which removal rate of Pb2+ was the highest. In brief, BP50-2 showed good performance on the removal of pigment, adsorption of heavy metals, and flocculation of particles.
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