Abstract
In this study, the aerial parts of Moricandia sinaica were evaluated for their in vivo analgesic, anti-inflammatory and antipyretic activities. The analgesic activities were examined using acetic acid-induced writhing, the hot plate test and the tail flick method. The anti-inflammatory and the antipyretic activities were evaluated using carrageenan-induced paw edema in rats and brewer’s yeast-induced pyrexia in mice, respectively. The aqueous fraction of the methanol extract (MS-3) showed to be the most bioactive among the other investigated fractions. At the dose of 500 mg/kg, the fraction (MS-3) showed a significant percentage inhibition of the carrageenan-induced edema by 52.4% (p < 0.05). In addition, MS-3 exhibited a significant inhibition of acetic acid-induced writhes by 44.4% and 61.5% (p < 0.001) at 250-mg/kg and 500-mg/kg doses, respectively. At 120 min post-treatment, the rat groups treated with MS-3 displayed statistically significant reduction in rectal temperature (p < 0.001) by 1.7 °C and 2.2 °C at 250- and 500-mg/kg doses, respectively. The phytochemical composition of the fraction (MS-3) was characterized by high-performance liquid chromatography-mass spectrometry (HPLC-PDA-MS/MS). Molecular docking studies demonstrated that the polyphenols identified in MS-3 revealed good binding energy upon docking to some target proteins involved in pain response and inflammation, such as the cannabinoid receptors CB1 and CB2, the fatty acid amide hydrolase (FAAH) and the cyclooxygenase COX-1 and COX-2 enzymes. Based on the findings from the present work, it could be concluded that the aerial parts extract of M. sinaica exerts potential analgesic, anti-inflammatory and antipyretic effects in rats.
Highlights
Inflammation is the body’s protective immune response that guards against cell injury and initiates tissue repair
Docking poses showed that the major compounds identified in the MS-3 fraction of M. sinaica were able to fit properly in the protein-binding pockets, affording diverse interactions with the amino acid residues and demonstrating appreciable free-binding energies reflected by minimum scoring function values compared to the reference ligands co-crystalized with the target proteins, as shown in sinaica docked to the cannabinoid receptors CB1 and CB2 and fatty acid amide hydrolase (FAAH) and cyclooxygenase (COX-1 and COX-2) enzymes
Major compounds identified in the M. sinaica most bioactive fraction (MS-3) were docked to some target proteins associated with analgesic and anti-inflammatory activities, namely the cannabinoid receptors CB1 and CB2, fatty acid amide hydrolase (FAAH), and cyclooxygenase COX-1 and COX-2 enzymes
Summary
Alsaid 2 , Mohamed A.O. Abdelfattah 4 , Riaz Ullah 2 , Mahmoud Emam 5,6, * , Abdelaziz Yasri 7 and Mansour Sobeh 7, *. Chemistry of Medicinal Plants Department, National Research Centre, Dokki, Giza 12622, Egypt. College of Pharmaceutical Science & Collaborative Innovation Center of Yangtze River Delta Region Green. Phytochemistry and Plant Systematics Department, National Research Centre, Dokki, Giza 12622, Egypt
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