Abstract
A new and efficient extraction procedure was proposed and used for the simultaneous extraction of four hydroxylated metabolites polycyclic aromatic hydrocarbons from urine samples. The extraction procedure was started by dissolving an organic polymer into a water-miscible organic solvent (iso-propanol) and its injection into the sample solution. The sorbent was re-precipitated in all parts of the solution as tiny particles and the analytes were adsorbed onto the sorbent. After that, the sorbent was separated and the adsorbed analytes were eluted by choline chloride: dichloroacetic acid deep eutectic solvent. The elution solvent was mixed with choline chloride-3,3-dimethyl butyric acid deep eutectic solvent and the mixture was applied in dispersive liquid-liquid microextraction procedure for more concentration of the analytes. After optimization, the method validation was followed according to International Council Harmonization guidelines and the results showed that wide linear ranges (26-500000ng/L) and low limits of detection (3.6-7.2ng/L) and quantification (11-26ng/L) were obtained. Satisfactory enrichment factors (435-475) and extraction recoveries (87-95%), and acceptable relative standard deviations (equal or less than 8.6%) were obtained. Finally, the introduced method was successfully applied for determination of the analytes in urine samples obtained from tobacco smokers.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
