A point mutation in the splice donor site of intron 7 in the αs2‐casein encoding gene of the Mediterranean River buffalo results in an allele‐specific exon skipping

Abstract

Table S1 Oligonucleotide primer sequences and positions Table S2 Thermal amplification programs for (a) RT-PCR (b) PCR and (c) AluI PCR-RFLP. Figure S1 Observed genotypes after AluI digestion of fragments obtained by PCR of the DNA region spanning the 7th exon and flanking regions of the Mediterranean river buffalo CSN1S2 gene. Figure S2 RP-HPLC chromatogram of individual buffalo milk samples. Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.