A platelet quality assessment scheme for comparing the performance of quality monitoring laboratories in the UK National Blood Service

Abstract

This exercise focused on performance of NBS quality monitoring establishments with respect to enumeration of low leucocyte and other quality indexes of platelet concentration. Paired identical leucodepleted platelet samples, spiked with WBC ( 20 cells/ μl ) in `vacuette' or `pouch' were assessed by participants ( n=20) on days 1, 2 and 5. For low WBC counting, all laboratories gave estimates within acceptable range (±25%) and good agreement between storage and assay methods was observed on days 1 and 2. Day 5 results showed greater variability. Under improved performance criteria (±15%), only one laboratory under-estimated at days 1 and 2. Similarly, other parameters demonstrated good agreement between storage methods on days 1 and 2. At day 5, mean results were often significantly different to previous days. Improved performance target (±15%) will allow identification of non-conformers.