A Plasmon-Enhanced Fluorescence Protein Microchip with High-Sensitivity for Multiple Islet Autoantibodies Measurements in Autoimmune Diabetes Diagnosis.

Abstract

Correct classification diagnosis of diabetes is critical for timely and appropriate treatment, which partially depends on the multiple islet autoantibodies measurement and combined analysis. This study reports plasmon-enhanced fluorescence protein microchip method, a fast, high-throughput, and high sensitive method, to measure circulating islet autoantibodies. This method can achieve islet autoantibodies combined analysis within a few hours by using a few microliters of serum. Combined islet autoantibodies analysis improved discriminatory ability, especially the diagnostic sensitivity. Furthermore, by applying this method, we found 4 out of 6 diabetes atypical patients who were clinically highly suspected of having autoimmune diabetes but had a negative result of glutamic acid decarboxylase antibody and insulinoma-associated protein 2 antibody by enzyme-linked immunosorbent assay were positive at least 1 out of 4 islet autoantibodies, largely reducing the rate of missed diagnosis of autoimmune diabetes and further validating its clinical value. This study shows the great clinical potentials of plasmon-enhanced fluorescence protein microchips.