Abstract
The human malaria parasite Plasmodium falciparum is responsible for the majority of malaria-related deaths. Tools allowing the study of the basic biology of P. falciparum throughout the life cycle are critical to the development of new strategies to target the parasite within both human and mosquito hosts. We here present 3D7HT-GFP, a strain of P. falciparum constitutively expressing the Green Fluorescent Protein (GFP) throughout the life cycle, which has retained its capacity to complete sporogonic development. The GFP expressing cassette was inserted in the Pf47 locus. Using this transgenic strain, parasite tracking and population dynamics studies in mosquito stages and exo-erythrocytic schizogony is greatly facilitated. The development of 3D7HT-GFP will permit a deeper understanding of the biology of parasite-host vector interactions, and facilitate the development of high-throughput malaria transmission assays and thus aid development of new intervention strategies against both parasite and mosquito.
Highlights
The human malaria parasite Plasmodium falciparum is responsible for the vast majority of malaria-related mortality
Examination of sporogonic development and exoerythrocytic schizogony has made use of Plasmodium berghei and P. yoelii rodent parasites constitutively expressing the green fluorescent protein (GFP) [2,3], these studies have considerably contributed to our knowledge of the complex life cycle of malaria parasites [4]
We here present the development of a stable transgenic P. falciparum parasite that constitutively expresses recombinant green fluorescent protein (GFP) in all life stages, facilitating observations in parasite biology and the understanding of intervention measures
Summary
The human malaria parasite Plasmodium falciparum is responsible for the vast majority of malaria-related mortality. Examination of sporogonic development and exoerythrocytic schizogony has made use of Plasmodium berghei and P. yoelii rodent parasites constitutively expressing the green fluorescent protein (GFP) [2,3], these studies have considerably contributed to our knowledge of the complex life cycle of malaria parasites [4]. We here present the development of a stable transgenic P. falciparum parasite that constitutively expresses recombinant green fluorescent protein (GFP) in all life stages, facilitating observations in parasite biology and the understanding of intervention measures.
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