Abstract
Abstract The construction of the plasmid pAF300, which contains the promoterless gene for chloramphenicol resistance fused to the broad host range conjugative vector pRK290, is described. Plasmid pAF300 could be used to identify transcription promoters by inserting DNA sequences in two cloning sites, HindIII and PstI. The vector efficiency was proved by cloning promoter sequences from Escherichia coli and Azospirillum brasilense which conferred chloramphenicol resistance to E. coli, A. brasilense and Pseudomonas putida.
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