Abstract

Abstract : A plaque technique for the assay of Rickettsia rickettsii is described. The method employs primary chick fibroblast cell cultures with a semisolid agar overlay. Plaques were observed after 6 days' incubation, and the titers correlated well with those obtained in embryonated eggs infected by the yolk-sac route. Identification of the rickettsia as the plaque-forming organism was accomplished by direct observation of rickettsial-like bodies in the monolayer lesions, specific fluorescent antibody staining, inhibition of plaques by antibiotics, sensitivity of the plaque to specific immune serum, and failure to cultivate, by a variety of methods, other microorganisms from the infected cells.

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