Abstract
The antigen–antibody complex (AAC) has novel functions for immunomodulation, encouraging the application of diverse quaternary protein structures for vaccination. In this study, GA733 antigen and anti-GA733 antibody proteins were both co-expressed to obtain the AAC protein structures in a F1 plant obtained by crossing the plants expressing each protein. In F1 plant, the antigen and antibody assembled to form a large quaternary circular ACC structure (~30 nm). The large quaternary protein structures induced immune response to produce anticancer immunoglobulins G (IgGs) that are specific to the corresponding antigens in mouse. The serum containing the anticancer IgGs inhibited the human colorectal cancer cell growth in the xenograft nude mouse. Taken together, antigens and antibodies can be assembled to form AAC protein structures in plants. Plant crossing represents an alternative strategy for the formation of AAC vaccines that efficiently increases anticancer antibody production.
Highlights
Antigen–antibody complexes (AACs) have been shown to regulate immune responses [1,2]
Transgenic plants carrying GAP and COP HCK and light chain (LC) genes were obtained by Agrobacterium-mediated transformation [25,26,27,28,29,30,31,32]
The GAP encoding gene was under the control of the enhanced cauliflower mosaic virus (CaMV) 35S promoter (Ca2p) and the tobacco etch virus 5 leader sequence (TEV)
Summary
Antigen–antibody complexes (AACs) have been shown to regulate immune responses [1,2]. Immunization using AACs has been used to enhance immunogenicity through the production of specific antibodies against immunogenic epitopes [3]. The Fc region of AAC can increase antigen uptake via the Fc receptor (FcR) that is present on antigen-presenting cells (APCs) and deliver the antigen for cross-presentation [5,6]. For this process, the roles of the Fc region of antibody have been reported, and they include interactions with both inhibitory FcγRIIB and activating FcγR found on the dendritic cells (DCs) and macrophages [7]. Antibody-derived immunomodulation induced by the formation of large protein complexes results in the induction of beneficial or protective immune responses [10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
