A PKC-β inhibitor treatment reverses cardiac microvascular barrier dysfunction in diabetic rats

Abstract

The PKC-β inhibitor ruboxistaurin (RBX or LY333531) prevents diabetic renal and retinal microvascular complications. However, the effect of RBX on diabetic cardiac microvascular dysfunction is still unclear. In this study, we aimed to investigate the effects and mechanisms of RBX treatment upon cardiac endothelial barrier dysfunction in high glucose states. We demonstrated RBX treatment suppressed high glucose induced PKC-βII activation and phosphorylation of β-catenin in vivo and in vitro experiments. Meanwhile, RBX treatment protected cardiac microvascular barrier function in diabetic animals and monolayer barrier function of cultured cardiac microvascular endothelial cells (CMECs), reproducing the same effect as PKC-βII siRNA. These results provide new insight into protective properties of PKC-β inhibitor against cardiac endothelial barrier dysfunction. PKC-β inhibitor RBX prevented chronic cardiac microvascular barrier dysfunction and improved endothelial cell–cell junctional function in high glucose states.