A pilot study to compare the activity of different bone morphogenetic protein bioimplants in vivo

Abstract

Event Abstract Back to Event A pilot study to compare the activity of different bone morphogenetic protein bioimplants in vivo Sean Peel1, 2, Katherine Lee1 and Cameron M. Clokie1, 2 1 University of Toronto, Oral & Maxillofacial Surgery, Canada 2 Induce Biologics Inc, Canada Background: The challenge in developing safe clinically and cost-effective recombinant bone morphogenetic protein-2 (BMP) implants is reducing the amount of BMP required. Current implants using absorbable collagen sponges (ACS) produce a burst release of BMP and require high doses of BMP to be effective. The aim of this study was to investigate using biphasic calcium phosphate (BCP) granules and calcium sulfate (CaS) granules in combination in P407 to deliver BMP. Methods: Different ratios of BCP to CaS granules were co-lyophilized with BMP. These were then combined with P407 gel with or without BMP. The implants were then placed in PBS buffer and the amount of BMP released evaluated by ELISA. Various BCP-CaS-P407 implants containing 40µg of BMP or ACS implants containing 80µg of BMP were implanted in muscle pouches of male CD-1 mice. At termination the amount of induced bone was quantified by microCT following correction for the presence of the implant and evaluated by histology. Statistical analysis was performed using ANOVA. Results: More bone was formed with implants containing CaS and/or BCP combined with poloxamer compared to ACS (p<0.05) even though the contained less BMP. A greater ratio of CaS granules within the implant resulted in more bone formation. The location of BMP affected bone formation with more bone formed when BMP was divided between the granules and poloxamer. Conclusions: BMP implants containing BCP, CaS and P407 may require less BMP than ACS implants to be effective. Funding was provided by Induce Biologics Inc Keywords: Bone Regeneration, growth factor, Bone graft Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Regenerative medicine: biomaterials for control of tissue induction Citation: Peel S, Lee K and Clokie CM (2016). A pilot study to compare the activity of different bone morphogenetic protein bioimplants in vivo. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02683 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sean Peel Katherine Lee Cameron M Clokie Google Sean Peel Katherine Lee Cameron M Clokie Google Scholar Sean Peel Katherine Lee Cameron M Clokie PubMed Sean Peel Katherine Lee Cameron M Clokie Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.