Abstract
The antibacterial and anti-inflammatory properties of lactoferricin have been ascribed to its ability to sequester essential iron. The objective of the study was to clone bovine lactoferricin (LFcinB) gene into PiggyBac Transposon vector, expression study in the bovine mammary epithelial stem cells (bMESCs) and also to determine the antimicrobial property of recombinant LFcinB against bovine mastitis-causing organisms. The PiggyBac-LFcinB was transfected into bMESCs by electroporation and a three fold of LFcinB secretion was observed in the transfected bMESCs medium by ELISA assay. Furthermore, the assessment of antimicrobial activity against mastitis causing pathogens Staphylococcus aureus and Escherichia coli demonstrated convincing evidence to prove strong antibacterial activity of LFcinB with 14.0±1.0 mm and 18.0±1.5 mm zone of inhibition against both organisms, respectively. The present study provides the convincing evidence to suggest the potential of PiggyBac transposon system to transfer antibacterial peptide into bMESCs or cow mammary gland and also pave the way to use bovine mammary gland as the bioreactors. Simultaneously, it also suggest toward commercial utilization of LFcinB bioreactor system in pharmaceutical industry.
Highlights
Infectious diseases constitute a pertinent concern in Human and Veterinary Medicine worldwide
The present study provides the convincing evidence to suggest the potential of PiggyBac transposon system to transfer antibacterial peptide into bovine mammary epithelial stem cells (bMESCs) or cow mammary gland and pave the way to use bovine mammary gland as the bioreactors
The amplified fragment of the synthetic gene was ligated into PiggyBac vector and the recombinant fragment was named as PiggyBac-bobine lactoferricin (LFcinB)
Summary
Infectious diseases constitute a pertinent concern in Human and Veterinary Medicine worldwide. Lactoferrin (LF) is a multifunctional protein found in bovine milk [4] with an antimicrobial properties: it is active against many Gram-negative and Gram-positive www.impactjournals.com/oncotarget bacteria [5], viruses [6], and various types of fungi and parasites [7, 8]. The antimicrobial peptides (AMPs) in modern pharmaceutical research play the vital role for human and veterinary medicine. These peptides show a broad spectrum of antimicrobial activity and can trigger specific defense responses in the host. The functional potential of antibacterial peptide LFcinB in inflammatory responses and the expression profiling in bovine mammary epithelial stem cells was investigated. The AMPs present the advantage of being derived from a harmless and inexpensive source and have an undeniable potential for use in medicine [10]
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