Abstract
ObjectivesThe study reveals a picture for M. gallisepticum (MG) and M. synoviae (MS) in poultry industry of Egypt. MethodsA total of 628 samples were collected from different species of diseased and apparently healthy poultryspeciesfrom 15 Egyptian governorates.The isolates identified based on mgc2and 16 s rRNAspecific primers for MG and MS respectively. Sequencing for two isolates usingmgc2gene. ResultsTwo hundred and five samples were microscopic fried egg colony.Using digitonin test, 160 samples were identified asgenusMycoplasma.Outofthe160 isolates, 86wereidentified by PCRas MG,while 39 were identified as MS. Sequencing reveals thatoneisolateMGC2-EGY1 was related totheEgyptian strainMG-EIS6-T-10, while, the other isolate MGC2-EGY2 was related totheIndian strain MG-MGS-9B. ConclusionThe study suggested that strictly applied programmes for the prevention and control of avian mycoplasmosis and confirmed the use of PCR on a large scale to help disease eradication programmes to limit economic losses in poultry farms.
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