Abstract
Electroporation is the electric-field induced formation of a voltage-dependent population of hydrophilic pores within a bilayer lipid membrane (BLM). This phenomenon can be observed as a transient increase in membrane conductance after the application of a voltage jump above a certain threshold. A very low current source is required to measure voltage fluctuations of a BLM due to voltage induced creation of dynamical pores during the electroporation. A current source able to drive a high-resistive load (on the order of 1012 Ω) by a constant current of 10 pA has been designed and realized. The current is maintained constant, with an uncertainty of 1.2% even when the resistive load fluctuates to 109 Ω over a very brief interval (10−3 s). These peculiar features have been obtained by designing a very simple circuit configuration, that gives an excellent match between the planned and the experimental behavior. The circuit bandwidth and the current precision are not the result of the circuit configuration, but are only limited by active components.
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