Abstract
Abstract— The phototransformation of native (124 kDa)oat phytochrome, Pr Pfr, Has been studied at 10d̀C by two laser/ two‐color flash photolysis. the overall PrPfr reaction yield did not vary with temperature within the range4–21d̀C. Foloeing the excitation of Pr with a single 15 ns laser flash at 650nm, the formation of Pfr was quantitavely measured in a time‐resolved experiment in the presence of a second 8 ns laser flash at 710 nm delayed from the initial flash. the second laser flash causes at 1.0 s after the initial laser flash a depletion of the uintermediate I700 as welll as a reduction of the Pfr absorption at 730 nm. The depletion of I700 correlates quantitavely with the reduction of Pfr formation. The absorpton spectra of I700 and of the following intermendiate, Ibi, were calculated assuming that the amount of Pr, which is photoconverted by a single laser, equals the amount of Pfr formed.
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