A Physical Map for the Heliothis zea SNPV Genome

Abstract

SUMMARY Restriction sites on the Heliothis zea nuclear polyhedrosis virus (HzSNPV) genome were mapped for the endonucleases BamHI, EcoRI, HindIII, SstI, XhoI and KpnI. XhoI-F was used as the zero-point of the physical map because it hybridized to the HindIII-V fragment which codes for the 3′ end of the polyhedrin gene of the Autographa californica nuclear polyhedrosis virus (AcMNPV). The DNA homology between eight AcMNPV cloned fragments and HzSNPV DNA was mapped using Southern hybridization and the locations of HzSNPV DNA sequences sharing homology with AcMNPV gene sequences are discussed.