Abstract
A PHOTOMETRIC NINHYDRIN METHOD FOR THE MEASUREMENT OF PROTEOLYSIS
Highlights
Since that portion of the synthetic substrate which is hydrolyzed is equal to each of the quantities of the hydrolytic products liberated, the color yield of a sample drawn during the course of enzymic hydrolysis may be represented as follows: rn0(a0- a) + m,a + m2a= D, [1]
Since the values for all samples are a function of the value for the 0 minute hydrolysis, i.e. Do of equation (Z), it is important that the latter value be determined for each hydrolysis mixture
Each sample withdrawn from the hydrolysis flask is added to a volumetric flask which has been previously partially filled with 1 per cent picric acid
Summary
Since that portion of the synthetic substrate which is hydrolyzed is equal to each of the quantities of the hydrolytic products liberated, the color yield of a sample drawn during the course of enzymic hydrolysis may be represented as follows: rn0(a0- a) + m,a + m2a= D,. In this equation, a0 is the initial substrate concentration, a is the concentration of each of the hydrolytic products, mois the slope of the line relating optical density to the concentration of the substrate. L-leucine, prepared in concentrations which would be obtained at various stages of hydrolysis when read in the photometer against Do, give optical densities which closely approximate the calculated hydrolysis curve
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
