A photoactivatable green-fluorescent protein from the phylum Ctenophora.

Abstract

You have accessMoreSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail Cite this article Haddock Steven H. D., Mastroianni Nadia and Christianson Lynne M. 2015A photoactivatable green-fluorescent protein from the phylum CtenophoraProc. R. Soc. B.2822015105520151055http://doi.org/10.1098/rspb.2015.1055SectionYou have accessRetractionA photoactivatable green-fluorescent protein from the phylum Ctenophora Steven H. D. Haddock Steven H. D. Haddock Google Scholar Find this author on PubMed Search for more papers by this author , Nadia Mastroianni Nadia Mastroianni Google Scholar Find this author on PubMed Search for more papers by this author and Lynne M. Christianson Lynne M. Christianson Google Scholar Find this author on PubMed Search for more papers by this author Steven H. D. Haddock Steven H. D. Haddock Google Scholar Find this author on PubMed Search for more papers by this author , Nadia Mastroianni Nadia Mastroianni Google Scholar Find this author on PubMed Search for more papers by this author and Lynne M. Christianson Lynne M. Christianson Google Scholar Find this author on PubMed Search for more papers by this author Published:07 August 2015https://doi.org/10.1098/rspb.2015.1055This article retracts the followingResearch ArticleA photoactivatable green-fluorescent protein from the phylum Ctenophorahttps://doi.org/10.1098/rspb.2009.1774 Steven H. D. Haddock, Nadia Mastroianni and Lynne M. Christianson volume 277issue 1685Proceedings of the Royal Society B: Biological Sciences16 December 2009In the years following the publication of ‘A photoactivatable green-fluorescent protein from the phylum Ctenophora’ [1], another research group [2] sequenced hydrozoan (Cnidaria) fluorescent proteins that were very similar to those we cloned from cDNA prepared from ctenophore specimens. We therefore now believe that the green-fluorescent proteins reported in our study are not from ctenophores, and were due to incorporation of cnidarian prey into the ctenophore tissues.Although our genes were cloned from two independent samples of mRNA taken years apart, the similarity of our sequences to those obtained from a siphonophore (Cnidaria, Hydrozoa) indicate that this mRNA was carried over from ingested material. This ctenophore species is known to prey on cnidarians, but we did not expect that mRNA would persist for as long as it did, and we regret our erroneous conclusion.What is remarkable about this situation is that the ctenophore, in addition to maintaining full-length mRNA for the fluorescent protein, also incorporated the proteins into its tissue to the extent that its bioluminescence emission was green-shifted. While the exact species of origin for these proteins is unknown, the photoactivatable properties of this fluorescent protein are as originally described, and it remains an interesting target for future research and application.Steven H. D. HaddockMonterey Bay Aquarium Research Institute, 7700 Sandholdt Road,Moss Landing, CA 95039, USAe-mail: [email protected]Nadia MastroianniAxxam SpA, San Raffaele, Biomedical Science Park, via Olgettina 58,20132 Milan, Italye-mail: [email protected]Lynne M. ChristiansonMonterey Bay Aquarium Research Institute, 7700 Sandholdt Road,Moss Landing, CA 95039, USAe-mail: [email protected]Footnotes© 2015 The Author(s)Published by the Royal Society. All rights reserved.References1Haddock SHD, Mastroianni N, Christianson LM. 2010A photoactivatable green-fluorescent protein from the phylum Ctenophora. Proc. R. Soc. B 277, 1155–1160. (doi:10.1098/rspb.2009.1774) Link, ISI, Google Scholar2Hunt ME, Modi CK, Aglyamova GV, Ravikant DVS, Meyer E, Matz MV. 2015Multi-domain GFP-like proteins from two species of marine hydrozoans. Photochem. Photobiol. Soc. 11, 637–644. (doi:10.1039/c1pp05238a) Crossref, ISI, Google Scholar Previous Article VIEW FULL TEXT DOWNLOAD PDF FiguresRelatedReferencesDetailsRelated articlesA photoactivatable green-fluorescent protein from the phylum Ctenophora16 December 2009Proceedings of the Royal Society B: Biological Sciences This Issue07 August 2015Volume 282Issue 1812 Article InformationDOI:https://doi.org/10.1098/rspb.2015.1055PubMed:26224709Published by:Royal SocietyOnline ISSN:1471-2954History: Published online07/08/2015Published in print07/08/2015 License:© 2015 The Author(s)Published by the Royal Society. All rights reserved. Citations and impact Subjectsbiochemistry Large datasets are available through Proceedings B's partnership with Dryad