A Photo-Responsive Porphyrin-Mn@Choles Complex for Bacteria Treatment

Abstract

Biocompatible photo-driven producers of singlet oxygen can inhibit the growth of drug-resistant bacteria and tumors. In order to develop bacteria targeting generator of singlet oxygen for tumor and bacterial treatment, a metal porphyrin liposome (Phy–Mn–Ls) was prepared by the metal coordination reaction and self-assembly of porphyrin compounds with bacteria targeting polymer (HS–PEG–chol). The photo-driven production of 1O2, binding with bovine serum protein (BSA) and lipase, toxicity to MCF-7 breast cancer cells and inhibitory effect on the growth of Escherichia coli have been investigated. Fluorescence analysis results show that Phy–Mn–Ls can bind to lipase, and it shows less effect on the conformation of BSA and is low cytotoxicity without irradiation. In particular, the good biocompatibility made Phy–Mn–Ls exhibit good photosensitive antibacterial activity and anti-tumor properties. The results demonstrate that the coordination of HS–PEG–chol with metal-phorphrin coodination is an effective way to develop bacteria targeting nano-complexes (Phy–Mn–Ls) for lipase affinity and photodriven bacteria treatment.