A Photo‐Cross‐Linking Strategy to Map Sites of Protein–Protein Interactions

Abstract

Clicked! Exemplified on a G-protein-coupled receptor (GPCR; see figure)/G-protein model system, a specific protein–peptide interaction was analyzed by using a G-protein-derived peptide containing p-ethynylbenzoyl-modified phenylalanine. Click chemistry with a biotin propyl azide probe enabled isolation of photo-cross-linked products and facilitated mass spectrometric analysis of the protein–peptide interaction sites. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.