Abstract

<p><em>Fumaria parviflora</em> Lamk., is a valued herb in Ayurvedic medicine and is used as Parpata by majority of Ayurvedic practitioners amongst the other plant sources mentioned under the same common name. It is found in many parts of India from Indo-Gangetic plain and Nepal down to the Nilgiri Mountains. The whole plant is diuretic, diaphoretic, aperient, laxative and anthelmintic. It is used as antipyretic, blood purifier and in skin disorders. In the present study, physico-chemical parameters were established for identification of the drug. Protopine and <em>β</em>-sitosterol were quantified by validated HPTLC method, developed using precoated silica gel plates as a stationary phase and toluene: ethyl acetate: diethyl amine (7: 2: 1) and toluene: methanol (9.4: 0.6) as a mobile phase respectively. It is a diffuse, annual herb with thin winged stem; alternate leaf<strong><em> </em></strong>finely divided into small, linear lanceolate segments, small white or pink flowers with purplish tips. Microscopically root can be characterized by the presence of centrally located diarch primary xylem encircled by wide secondary xylem occupying major area and a narrow cork; stem by collenchymatous hypodermis, vascular bundle capped with lignified pericyclic fibres and hollow pith; leaf by vascular bundles with groups of sclerenchyma underneath the phloem and narrow spongy parenchymatous lamina. Powder<strong><em> </em></strong>can be typified by xylem vessels with varied thickening, lignified and thick walled testa and spherical pollen grains. The plant was found to be rich in alkaloids. The amount of protopine and <em>β</em>-sitosterol were found to be 0.47 – 0.50% w/w and 0.23 – 0.26% w/w. The quality parameters and HPTLC method developed would serve as useful gauge in standardization of <em>Fumaria parviflora</em>.</p>

Highlights

  • Plant MaterialFully-grown, flowering plants of F. parviflora were collected from Jammu in the month of January 2012

  • IpFoummoaeariarenpiaforrvmiflisorCahaLoasmiskc.,laiismaedvianluIneddiahnetrrbadiintioAnyaul mrveeddicicalmpreadcitcicineetoabneduissefuusleind tahse Ptraeraptmateantbyofmepaijloepristyyaonfd Ayurvedic nepurraocltoigtiiocanledrisoarmdeorsn.gInstththeeproetsheentr sptuladnyt, psroeutrrecaetsmmenetnetfifoencteodf umnedthearntohliec seaxtmraectcoofmIpmomonoenaarmeneif.oIrtmiiss foonuenpdileinpsmy any parts anodf pInsydcihaofsrisowmasInedvaol-uGaatendgientircodpelnatins uasnindg sNtaenpdaalrdopwroncetdourtehse

  • Karuna Modi. et al 3 bearing lateral wiry rootlets; erect, longitudinally wrinkled, often branched 20-30 cm long stem with 4 to 5 winged projections; alternate, exstipulate leaf that is finely divided into narrow flat segments, each segment being broad oblong or linear lanceolate, 2 to 3 cm in length and 1 to 2 mm in width with acute or subacute apex, 2 to 4 cm long twisted petiole, sheathing at base; small white or pink flowers with purplish tips, in terminal inflorescence; indehiscent, tiny, sub-globose and externally faintly rugose fruits and globose minute seeds

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Summary

Plant Material

Fully-grown, flowering plants of F. parviflora were collected from Jammu in the month of January 2012. The plants collected were authenticated by taxonomist of Gujarat University, Ahmedabad, Gujarat. Voucher specimen sample (LM 632) was deposited at the Department of Pharmacognosy, L. The plant material was cleaned, dried, powdered to 60 # and used for the present study

Pharmacognostical Studies
Chromatographic Conditions
Calibration Curve
Quantification of Protopine and β-sitosterol in Extract
Macroscopical Evaluation
Microscopical Evaluation
Powder
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