Abstract
Sijunzi Decoction (SJZD) is a formula used for the treatment of spleen deficiency and gastrointestinal diseases in Traditional Chinese Medicine. Polysaccharides are reported to be the main components of SJZD responsible for its bio-functions. However, highly purified and clearly characterized polysaccharides from SJZD are not well described. Here we obtained a purified polysaccharide (SJZDP-II-I) from SJZD using ion exchange chromatography and gel filtration. Structure analysis by FT-IR and NMR identified SJZDP-II-I as a typical pectic polysaccharide with homogalacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and II as side chains. In vitro studies indicated that SJZDP-II-I treatment could significantly enhance the total antioxidant capacity of SW480 cells, resulting from the promoted expressions of antioxidant enzymes and their master regulator PGC-1α, which would be valuable for further research and applications.
Highlights
A Pectic Polysaccharide from Sijunzi DecoctionChao Huang 1,3,† , Zhongkai Zhu 2,† , Xiyue Cao 1,3 , Xingfu Chen 5 , Yuping Fu 2,3 , Zhengli Chen 1,3 , Lixia Li 2,3 , Xu Song 2,3 , Renyong Jia 3 , Zhongqiong Yin 2,3 , Gang Ye 2 , Bin Feng 4 ID and Yuanfeng Zou 2,3, * ID
As a conventional Traditional Chinese Medicine Formula (TCMF), Sijunzi Decoction (SJZD), consisting of four crude herbs (Panax ginseng, Atractylodes macrocephala, Poria cocos and Glycyrrhiza uralensis) in the ratio of 3:3:3:2, has been widely applied for over one thousand years in the treatment of gastrointestinal diseases, such as stomach aches, rugitus, nausea, vomiting and diarrhea [1,2].Modern pharmacological studies have further revealed its potential effects on the immune system activation and cancer treatment [1,3,4]
SJZDP-II-I was chosen for structure elucidation as the other two fractions showed no activity in promoting the antioxidant defenses of SW480 cells
Summary
Chao Huang 1,3,† , Zhongkai Zhu 2,† , Xiyue Cao 1,3 , Xingfu Chen 5 , Yuping Fu 2,3 , Zhengli Chen 1,3 , Lixia Li 2,3 , Xu Song 2,3 , Renyong Jia 3 , Zhongqiong Yin 2,3 , Gang Ye 2 , Bin Feng 4 ID and Yuanfeng Zou 2,3, * ID.
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