Abstract
A PCR detection method for mutations in receptor protein genes from Busseola fusca midgut material potentially involved in Bt-resistance Busseola fusca (Fuller) (Lepidoptera: Noctuidae) is a major insect pest to Bt-maize in South Africa. No study has yet been done to determine the molecular mechanism of B. fusca resistance to Cry1Ab. Aminopeptidase N (1-6), cadherin and alkaline phosphatase receptor-protein genes were amplified and sequences of PCR products were determined. BLAST searches showed similarity to the respective receptor-protein genes. Mutations can thus be identified by comparing sequences of receptor-protein genes from resistant and susceptible larvae.
Highlights
Note: This paper was initially delivered at the School of Environmental Sciences and Development of the North-West University, Potchefstroom Campus, South Africa on 05 October 2012
A PCR detection method for mutations in receptor protein genes from Busseola fusca midgut material potentially involved in Bt-resistance
No study has yet been done to determine the molecular mechanism of B. fusca resistance to Cry1Ab
Summary
Note: This paper was initially delivered at the School of Environmental Sciences and Development of the North-West University, Potchefstroom Campus, South Africa on 05 October 2012. ’n PKR-opsporingsmetode vir mutasies in proteïenreseptorgene vanaf Busseola fusca-ingewande, moontlik betrokke by Bt-weerstandbiedendheid & Bezuidenhout, C.C., 2014, ‘’n PKRopsporingsmetode vir mutasies in proteïenreseptorgene vanaf Busseola fusca-ingewande, moontlik betrokke by Btweerstandbiedendheid’, Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie 33(1), Art. Busseola fusca (Fuller) (Lepidoptera: Noctuidae) is a major insect pest to Bt-maize in South Africa.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
