A particular binding of L cell-grown Sendal virus by host L cells. (Growth characteristics of myxoviruses in tissue culture. 5th).

Abstract

Egg adapted Sendai virus is fully infectious to both chick embryos and Earle's L cell. In the latter system, reproduction of egg infectious particles has been already proved. However, the L cell-grown virus differs from parent virus in its non-infectiousness, when L cell was used again for its reproduction. Thus the study was conducted to reveal the characters of this L cell-grown virus. First of all, it was shown that adsorption of this particular virus to the L cell did occur in the same efficiency as that of egg adapted virus when tested in the cold. Possible adsorption to the L cell was again confirmed by testing the interfering activity of the L cell infected with L cell-grown virus at 36°C, where HA production of challenged egg virus was used as an indicator. However, adsorption feature of the L cell-grown virus to the L cells was also shown to be quite characteristic. Adsorbed virus once proved in the cold was found to be elutable at 36°C, as was the case between a myxovirus and chicken red blood cells. Furthermore, this kind of adsorption was found to be eliminated by treatment with immune serum prepared against Sendai virus. Thus the L cell-grown virus was anticipated to be not equipped enough as egg adapted virus to invade the L cell again. Even when large amount of L-cell grown harvest was purified by density gradient centrifugation, most dense particles highly infectious to chick embryos failed to do so. Receptor destroying activity of this variant, so far examined by biological procedure, was not different from that of parent virus.