Abstract
In skeletal surgical procedures, bone regeneration in irregular and hard-to-reach areas may present clinical challenges. In order to overcome the limitations of traditional autologous bone grafts and bone substitutes, an extrudable and easy-to-handle innovative partially demineralized allogenic bone graft in the form of a paste has been developed. In this study, the regenerative potential of this paste was assessed and compared to its clinically used precursor form allogenic bone particles. Compared to the particular bone graft, the bone paste allowed better attachment of human mesenchymal stromal cells and their commitment towards the osteoblastic lineage, and it induced a pro-regenerative phenotype of human monocytes/macrophages. The bone paste also supported bone healing in vivo in a guide bone regeneration model and, more interestingly, exhibited a substantial bone-forming ability when implanted in a critical-size defect model in rat calvaria. Thus, these findings indicate that this novel partially demineralized allogeneic bone paste that combines substantial bone healing properties and rapid and ease-of-use may be a promising alternative to allogeneic bone grafts for bone regeneration in several clinical contexts of oral and maxillofacial bone grafting.
Highlights
In skeletal surgical procedures, bone regeneration in irregular and hard-to-reach areas may present clinical challenges
Allogeneic bone grafts are mainly used in powder or block forms, they exhibit a lack of versatility and/or handling, especially in specific indications related to pre-implant surgery, such as sinus floor elevation, guided bone regeneration in horizontal/vertical augmentation, and alveolar socket preservation
The purpose of this study was to assess the effects of the bone paste in vitro (1) on the attachment and osteoblastic commitment of primary human mesenchymal stromal cells from bone marrow, (2) on the polarization of primary human monocytes isolated from circulating blood, and (3) to test and validate the in vivo capacity of this innovative bone paste to support and promote bone healing in two preclinical models: a guided bone regeneration (GBR) and a critical size defect (CSD) model in rat calvaria to mimic intramembranous bone healing of oral and maxillofacial defects
Summary
Bone regeneration in irregular and hard-to-reach areas may present clinical challenges. Allogeneic bone grafts are mainly used in powder or block forms, they exhibit a lack of versatility and/or handling, especially in specific indications related to pre-implant surgery, such as sinus floor elevation, guided bone regeneration in horizontal/vertical augmentation, and alveolar socket preservation. To address these unmet clinical needs, a novel ready-to-use, easy-to-handle, extrudable, and moldable human bone graft for bone regenerative medicine has been developed[12]. The present work aimed to provide a thorough assessment of the bone healing capacity of this allogeneic bone paste compared with an allogeneic particular bone graft before its partial demineralization
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
