Abstract
A new method permitting the estimation of estradiol, testosterone and 5α-dihydrotestosterone in the same plasma sample is presented. This is made possible by a 20 h overflow paper chromatography system used to purify the plasma extract prior to steroid analysis. Estradiol is well separated from estrone as is testosterone from 5α-dihydrotestosterone. The method has low blank values, good agreement between duplicates, acceptable recovery through the extraction and purification and a good recovery of steroids added to plasma prior to extraction. Plasma levels of steroids measured using this method agree well with the values obtained by other methods.
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