Abstract
A µPAD to determine the total polyphenol content (TPC) by Folin-Ciocalteau (FC) method, and antioxidant capacity by ABTS method, coupling an in-situ pretreatment zone with Carrez reagents is proposed. Conditions of FC, K2S2O8, ABTS reagents concentrations, pH, and incubation were established using 2k factorial designs. The proposed µPAD showed linearities upper than 0.99 in ranges 0.150–2.000 mg L−1 and 0.100–1.000 mg L−1 for FC and ABTS methods, respectively, detection limits were lower than 0.135 mg L−1, precision lower than 13.47 %, and recoveries between 82.27 % and 114.60 %. Ten different food samples were analyzed to demonstrate the applicability of the µPAD, and the results were compared with those obtained by the batch methods, showing no significant differences (p > 0.05). The µPAD in-situ sample treatment allowed removing interferences before the TPC and ABTS detection avoiding overestimation of the results.
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