Abstract
<p>The objective of this study was to identify pancreatic lipase inhibitory active ingredients of mango leaves, and to examine a relationship between leaves maturation and pancreatic lipase inhibitory activity. A methanolic extract of old dark green mango leaves (OML-ext) showed a porcine pancreatic lipase inhibitory activity. The pancreatic lipase inhibitory activity of OML-ext was attributable to 3-C-<em>β</em>-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (<strong>2</strong>) and mangiferin (<strong>1</strong>). The pancreatic lipase inhibitory activity of young mango leaf extract was superior to that of old leaf extract. It was suggested that the activity is correlated with the content of <strong>2</strong> in these extract. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for the preparation of ingredients with lipase inhibitory activity.</p>
Highlights
Obesity is recognized as a major life style disorder especially in developing countries and it is prevailing at an alarming speed in the world due to fast food intake, industrialization, and reduction of physical activity (Cairns, 2005).Pancreatic lipase is a key enzyme for lipid absorption by hydrolysis of total dietary fats (Seyedan et al, 2015)
Silica gel column chromatographic fractionation of old mango leaf extract (OML-ext) gave active fractions eluted with CHCl3/MeOH 30:1 v/v, 5:1 v/v and 0:10 v/v, and inactive fractions eluted with CHCl3/MeOH 10:0 v/v
A part of the pancreatic lipase inhibitory activity of OML-ext is attributable to these two compounds
Summary
Pancreatic lipase is a key enzyme for lipid absorption by hydrolysis of total dietary fats (Seyedan et al, 2015). In order to find new pancreatic lipase inhibitors from natural resources, screening of plant extracts has been considered as one of strategies. Several extracts of the plants, such as chokeberry fruit (Sosnowska et al, 2015), Nelumbo nucifera leaves (Liu et al, 2013), Coffea arabica seeds (Patui et al, 2014) and Panax japonicus rhizomes (Han et al, 2005), have been reported to have lipase inhibitory activities. Lipid metabolic enzyme inhibitory (Moreno et al, 2006) and cholesterol esterase inhibitory activities (Gururaja et al, 2015) of mango leaves have been reported, pancreatic lipase inhibitory activity of young mango leaves has never been examined
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