Abstract
Sucrose is the main saccharide used for long-distance transport in plants and plays an essential role in energy metabolism; however, there are no analogues for real-time imaging in live cells. We have optimised a synthetic approach to prepare sucrose analogues including very small (≈50 Da or less) Raman tags in the fructose moiety. Spectroscopic analysis identified the alkyne-tagged compound 6 as a sucrose analogue recognised by endogenous transporters in live cells and with higher Raman intensity than other sucrose derivatives. Herein, we demonstrate the application of compound 6 as the first optical probe to visualise real-time uptake and intracellular localisation of sucrose in live plant cells using Raman microscopy.
Highlights
Sucrose metabolism is one of the main processes that regulates the development, growth and functioning of higher plants.[1]
Our results show that probe 6 is only partially degraded by sucrose invertase
We examined the utility of compound 6 for realtime imaging of sucrose trafficking in live plant cells
Summary
A Palette of Minimally Tagged Sucrose Analogues for RealTime Raman Imaging of Intracellular Plant Metabolism. Citation for published version: De Moliner, F, Knox, K, Gordon, D, Lee, M, Tipping, WJ, Geddis, A, Reinders, A, Ward, J, Oparka, K & Vendrell, M 2021, 'A Palette of Minimally Tagged Sucrose Analogues for RealTime Raman Imaging of Intracellular Plant Metabolism', Angewandte Chemie International Edition.
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