Abstract
A pair of new phenolic acid stereoisomers, (R)-norsalvianolic acid L (1) and (S)-norsalvianolic acid L (2), was isolated from the Danshen Injection (lyophilized powder). The structural elucidation and stereochemistry determination were achieved by spectroscopic and chemical methods including 1D, 2D NMR (1H-1H COSY, HSQC and HMBC) and circular dichroism experiments. Their antioxidant activities were assessed by the DPPH· and ABTS·+ scavenging methods in vitro with microplate assay. The IC50 values of 1 were 6.9 and 9.7 μM respectively, which was close to the control salvianolic acid B (7.8 and 7.1 μM respectively), while the IC50 values of isomer 2 were 27.1 and 25.3 μM, respectively.
Highlights
In China Salviae miltiorrhizae Radix et Rhizoma, called Danshen, is an important commonly used Traditional Chinese Medicine
To elucidate the effective substances responsible for traditional function and indication, the phenolic constituents of Danshen Injection were chemically investigated, and their radical scavenging capacities were measured by DPPH and ABTS assays
We report the isolation, structural identification and antioxidant activity of the stereoisomers (R)-norsalvianolic acid
Summary
In China Salviae miltiorrhizae Radix et Rhizoma, called Danshen, is an important commonly used Traditional Chinese Medicine. It activates and promotes blood circulation, and is widely used for the treatment of coronary heart diseases, hepatitis and dysmenorrhea [1]. To elucidate the effective substances responsible for traditional function and indication, the phenolic constituents of Danshen Injection (lyophilized powder) were chemically investigated, and their radical scavenging capacities were measured by DPPH and ABTS assays. We report the isolation, structural identification and antioxidant activity of the stereoisomers (R)-norsalvianolic acid.
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