Abstract
Spring viremia of carp virus (SVCV) is a high pathogenic and threatening virus in cyprinids culture because of no therapeutic measures. In the present study, we designed and synthesized a safer derivative, 7-N-(p-methylphenyl sulphonylamine)-4-methylcoumarin (LMC1011). The present data showed that LMC1011 increased the cell viability of SVCV-infected epithelioma papulosum cyprini (EPC) cells by 63% and inhibited the virus replication in vitro over 90% at 300 μM. Mechanistically, it is activation of intracellular antiviral signaling pathways to affect the virus internalization. For in vivo study, continuous oral injection of LMC1011 increased the survival rate of SVCV-infected common carp by 55%, and significantly reduced the viral loads in kidney and spleen. Additionally, pretreatment of LMC1011 upregulated the expression of IFN-γ2b, IFN-I, ISG15 and Mx1 genes to effectively improve the resistance of common carp against SVCV infection. Overall, our results suggest that application of LMC1011 may stimulate antiviral immune response of fish to effectively control SVCV infection in aquaculture.
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