Abstract
A simple, one-step method for covalent bond immobilization of biomolecules on silica operated in water is described. In the approach, an NHS-ester linked methallylsilane is utilized as a bifunctional linker to couple the biomolecule to the silica surface. Weak organic acid such as acetic acid activates the silica surface enough to react with bifunctional linker without destroying activity of biomolecule.
Highlights
While signi cant advances have been made in fabricating and utilizing immobilized systems, the development of novel techniques for efficient immobilization of biomolecules on inorganic supports requires additional attention.[1]
To explore the novel one-step protocol, the designed NHS-ester linked methallylsilane 1 is utilized as a bifunctional reagent
bovine serum albumin (BSA)@Si, generated using the one-step method has a much higher degree of loading (135 mg) than does g-BSA@Si (28 mg). Another enzyme explored in this effort is Candida antarctica lipase B (CAL B), which is known to display excellent levels of enantioselectivity in kinetic resolution (KR) promoting transesteri cation processes.[11]
Summary
While signi cant advances have been made in fabricating and utilizing immobilized systems, the development of novel techniques for efficient immobilization of biomolecules on inorganic supports requires additional attention.[1]. A recent investigation was conducted to explore a two-step method involving immobilization on nonmodi ed mesoporous silica of pre-made, chemically modi ed glucose oxidase (GOx) promoted by transition metal catalysts such as Sc3+ (Fig. 1b).[4] While this two-step protocol provides an expedient platform to prepare GOx-functionalized silica, the scope of biomolecules using this protocol was determined to be narrow (vide infra).
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