Abstract
A rapid one-step assay for intestinal peptide hydrolase activity in mucosal homogenates, and in cytosol and particulate fractions prepared from homogenates is described. Peptide hydrolysis, and estimation of liberated l-amino acids by use of l-amino acid oxidase, peroxidase, and a chromogen are achieved by a single incubation step. The most suitable peptide substrates are dipeptides composed of one amino acid which reacts strongly and rapidly with the l-amino acid oxidase reagent and one which does not react. Brush border peptide hydrolase activity may also be estimated with this assay by performing the incubation in the presence of p-hydroxymercuribenzoate.
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