Abstract
The Streptomyces virginiae barA gene encodes a specific receptor protein for virginiae butanolide (VB), one of the γ-butyrolactone autoregulators of Streptomyces species. By homologous recombination, a barA null strain was constructed to clarify the in vivo function of BarA protein in S. virginiae. The Δ barA mutant showed no difference in terms of growth, but lost VB production and produced virginiamycin 7 h earlier than the wild-type strain. These results indicated that, phenotypically, BarA protein acts negatively in virginiamycin biosynthesis and positively in VB biosynthesis. Furthermore, Northern (RNA) blot analysis of the Δ barA mutant revealed that transcription of the BarA target gene ( barB) was derepressed, confirming that BarA acts as a transcriptional repressor in S. virginiae.
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