Abstract
In the present study, a Nucleopolyhedrovirus (NPV) was isolated from laboratory-reared larva of the Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Under the light microscope, high numbers of polyhedral inclusion bodies (PIBs) formed by the virus were found in hemolymph, tracheae, and the midgut. The PIBs measured 1.97 ± 0.46 (1–3.04) µm (n = 50). In cross-sections of PIBs, the virions contain a high number of nucleocapsids (1–28), and the viral particles were 190–270 × 30–40 nm. Some diamond-shaped inclusion bodies, like spindles, which did not include virions, were also observed. This virus was isolated and characterized for the first time from E. kuehniella.
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