Abstract
Chromosomal DNA methylation patterns were determined in the grasshopper Eyprepocnemis plorans by in situ digestion with MspI and HpaII. While no methylated regions were observed in standard chromosomes, the B chromosome was methylated in the distal third of its long arm. In this zone the B chromosome had an active nucleolus organizer region (NOR) in a male carrying a centric fusion between the B and the longest autosome, and it was not methylated. This NOR, however, was never observed in the active form in nonfused B chromosome, possibly because of methylation of this B chromosome region.
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