Abstract
A nuclear polyhedrosis virus (CrMNPV) has been isolated from larvae of the obliquebanded leafrollerChoristoneura rosaceana(Harris) collected near St.-Quentin in northwest New Brunswick, Canada. Restriction endonuclease digestion–gel electrophoresis of this virus, using a number of different endonucleases and whole CrMNPV genome Southern hybridization, have shown it to be different but similar to the equivalent virus from the spruce budwormChoristoneura fumiferana(Clem.) (CfMNPV). The polyhedrin gene of CrMNPV has been isolated, cloned, and sequenced and shown to have a predicted amino acid sequence only two amino acids different from the CfMNPV polyhedrin. In bioassays against spruce budworm larvae, CrMNPV had little pathogenicity to that host.
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