A novel Wulff‐type boronate acid‐functionalized magnetic metal‐organic framework imprinted polymer for specific recognition of glycoproteins under physiological pH

Abstract

Boronate affinity molecularly imprinted materials have been widely used for the separation of glycoproteins under alkaline conditions that is not conducive to the structural stability of the protein. In this work, a kind of novel molecularly imprinted polymer (MIP/TBA/MOF@Fe3 O4 ) was prepared via grafting self-assembled molecular team of boronic acids on the surface of the magnetic metal-organic framework core. The teamed boronate affinity was formed by 2-mercaptoethylamine and 4-mercaptophenylboronic acid for specific separation of glycoproteins under physiological pH (pH7.4). The obtained nanoparticles show high binding capacities (337.8mg/g), fast adsorption equilibrium time (20min), and good specificity (imprinting factor, 4.52) for glycoproteins under physiological pH. Furthermore, the prepared imprinted polymer still shows good adsorption capacity for glycoprotein after five times of repeated use, and its adsorption capacity only dropped by 4.7%. More importantly, the prepared nanoparticles have good potential to adsorb glycoproteins from real biological samples.