Abstract
Motivation: Accurate identification and delineation of promoters/TSSs (transcription start sites) is important for improving genome annotation and devising experiments to study and understand transcriptional regulation. Many promoter identifiers are developed for promoter identification. However, each promoter identifier has its own focuses and limitations, and we introduce an integration scheme to combine some identifiers together to gain a better prediction performance. Result: In this contribution, 8 promoter identifiers (Proscan, TSSG, TSSW, FirstEF, eponine, ProSOM, EP3, FPROM) are chosen for the investigation of integration. A feature selection method, called mRMR (Minimum Redundancy Maximum Relevance), is novelly transferred to promoter identifier selection by choosing a group of robust and complementing promoter identifiers. For comparison, four integration methods (SMV, WMV, SMV_IS, WMV_IS), from simple to complex, are developed to process a training dataset with 1400 se- quences and a testing dataset with 378 sequences. As a result, 5 identifiers (FPROM, FirstEF, TSSG, epo- nine, TSSW) are chosen by mRMR, and the integration of them achieves 70.08% and 67.83% correct prediction rates for a training dataset and a testing dataset respectively, which is better than any single identifier in which the best single one only achieves 59.32% and 61.78% for the training dataset and testing dataset respectively.
Highlights
IntroductionA short DNA sequence, is the binding site of RNA polymerases
Promoter, a short DNA sequence, is the binding site of RNA polymerases
Four integration methods Simple Majority Voting (SMV), Weighted Majority Voting (WMV), Simple Majority Voting plus Identifier Selection (SMV_IS) and Weighted Majority Voting plus Identifier Selection (WMV_IS), from simple to complex, are developed to process a training dataset with 1400 sequences and a testing dataset with 378 sequences
Summary
A short DNA sequence, is the binding site of RNA polymerases. It determines the transcription start site (TSS). After RNA polymerase binding to a promoter, the promoter initiates the transcription and indicates where the transcription should start. In order to be recognized by the RNA polymerases, the structure of promoters is rather stable, e.g. in eukaryotic genome, many promoters contain TATA box, which can help locate promoters by searching TATA sequences. Besides TATA box, functional motifs, oligonucleotide composition and compositional features are used for promoter identification [1,2,3,4,5,6,7,8]. This paper investigates a novel way to combine some promoter identifiers together to improve the identification rate
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