Abstract
A thermometer-type visual sensor for glutathione (GSH) sensing was developed with stimulus-responsive fluorescent hydrogel which was obtained by using 5, 6-bicarboxylic fluorescein crossli`nked partly ammoniated polyacrylamide. Various experimental parameters such as the particle size of hydrogel, buffer solution and swelling time were optimized. It is accessible to measure the volume change of hydrogel with the sensor by reading the graduation on a pipette like thermometer with naked eye. The concentration of the GSH depended on the volume in a certain range as the signal. Satisfactory agreements between the sensor and HPLC results for atuomolan tablet assays indicated the capability of the thermometer-type sensors for the analysis of real samples. These findings proved the utility of stimulus-responsive, intelligent hydrogel and the suitability of thermometer-style visual sensor design for quantitative assays.
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