Abstract
Objective: Adult stem cells offer the possibility of tissue regeneration following injury. However, for most stem cells, markers are not available for cell identification and purification. Accordingly, alternative approaches for stem cell identification have been developed. Hematopoetic stem cells can be enriched by exploiting their ability to home to the bone marrow of irradiated mice. In this study, we describe the development of a similar technique to isolate and enrich a population of adult spermatogonial stem cells for germ cell transplantation. Our ultimate goal is to develop a novel technique for the purification of adult spermatogonial stem cells to be used for transplantation as a therapy for primary or secondary male infertility
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