Abstract
We have developed and validated a novel technique that allows for a relatively quick and efficient isolation of embryonic tissue from the yolk in the rainbow trout, Oncorhynchus mykiss. After removal of the chorion, the yolk is first dissolved in an imidazole-KCl buffer and separated from the embryo proper and yolk sac (tissues) by centrifugation. SDS gel was used to identify proteins of 165, 48, 35 and 10 kDa unique to the embryonic tissue and a 6 kDa protein unique to the yolk. Using protein analysis and enzyme activity measurements (lactate dehydrogenase and glutamate dehydrogenase) of each fraction, we have determined that cross contamination is less than 10%. This new method will facilitate biochemical studies of the embryo and yolk that were previously very time consuming.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
