Abstract
The tapetum, a thin layer of cells in the anther, is important to the process of male gametogenesis. However, only a few of its functioning genes have been reported. Here we isolated a genomic clone,gNtA37, that showed tapetum-preferential expression. Transcript could be detected from the microspore mother-cell stage until the time that microspores were released. It coded a protein of 245 amino acids, with an intervening sequence of 176 nucleotides. This clone also carried a 5′ upstream region of 840 nucleotides and a 3′ downstream region of 430 nucleotides. Database searches for the open reading frame did not reveal any significant homology to previously reported nucleotide sequences or polypeptide sequences.
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