Abstract
A new species of torque teno mini virus, named TTMV-222, was detected in gingival tissue from periodontitis patients using a viral metagenomics method. The 2803-nucleotide genome of TTMV-222 is closely related to TTMV1-CBD279, with 62.6% overall nucleotide similarity. Genetic analyses of the new virus genome revealed a classic genomic organization but a weak identity with known sequences. The prevalence of TTMV-222 in the periodontitis group (n = 150) was significantly higher than that in the healthy group (n = 150) (p = 0.032), suggesting that the new virus may be associated with inflammation in chronic periodontitis patients. However, this finding requires further investigation.
Highlights
The causative agents of periodontal disease are generally thought to be periopathogenic bacteria, the pure bacterial pathogenicity theory cannot cover all of the manifestations of periodontal diseases
Increasing evidence support a role for pathogens other than bacteria in the pathogenesis of periodontal disease[7,9,10]
Published evidence has strongly suggested that Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) are implicated in the pathogenesis of periodontal disease[2,5,7,9,10]
Summary
A case control study was conducted to investigate the association between the prevalence of the newly identified virus and chronic periodontitis. We enrolled 150 chronic periodontitis patients (mean age 45) and 150 periodontal healthy participants (mean age 40) in a case control study. Based on the amplified full-length sequence, primers (DL1 5′-T*G*AGTGAAACCACCGAAGTC-3′, DR1 5′-C*G*TTACTTGTTGTCCACCAG-3′, DL2 5′-ACCACGGATTATTCTGCGGC-3′, and DR2 5′-AAAAGACCATGCTCCCCCTC-3′) were designed to screen the samples from the chronic periodontitis patients and periodontally healthy subjects for the novel TTMV.
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