Abstract
A novel smartphone-based colorimetric biosensor based on enzyme-inorganic hybrid nanoflowers was applied for ultrasensitive determination of of hydrogen peroxide (H2O2). Horseradish peroxidase-inorganic hybrid nanoflowers (HRP-Cu3(PO4)2⋅3H2O HNFs) were facilely synthesized by a one-pot incubation using HRP and Cu3(PO4)2⋅3H2O as organic and inorganic components, respectively. Characterization results revealed HRP molecules were well immobilized in HRP-Cu3(PO4)2⋅3H2O HNFs via self-assembly. The results of smartphone-based colorimetric assay successfully demonstrated the good sensing performance of HRP-Cu3(PO4)2⋅3H2O HNFs. The linear detection range of H2O2 was from 5 to 500 μM, and the detection limit was 0.5 μM. Further, the proposed assay showed good anti-interference ability and high sensitivity for H2O2 detection owing to the high specificity of HRP and excellent accuracy of color parameters obtained by Color Detector software, respectively. In addition, HRP-Cu3(PO4)2⋅3H2O HNFs had desirable storage stability and reusability, and this colorimetric system can realize the H2O2 detection in tap water, which indicated the good applicability of HRP-Cu3(PO4)2⋅3H2O HNFs in real sample.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
